Vol. 75 (2) 2023

ARTICLES

Contents of Zn, Cu, Mn and Cd in soil, plants and Turkish snails Helix lucorum Linnaeus, 1758 in Skopje, Republic of North Macedonia

Trajche Mitev1, Ivaylo K. Dedov2 & Slavcho Hristovski3

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1Dekons Ema Consultancy, 1000 Skopje, Republic of North Macedonia; E-mail: trajcho.mitev@gmail.com
2Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113 Sofia, Bulgaria;
E-mail: idedov@gmail.com
3Institute of Biology, Faculty of Natural Sciences and Mathematics, University „St. Cyril and Methodius“, Skopje,
Republic of North Macedonia; E-mail: slavco_h@pmf.ukim.mk

https://doi.org/10.71424/azb75.2.002588

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Abstract
Heavy metal pollution of the environment is especially apparent within or around urban areas and around industrial facilities. This paper deals with the concentration of zinc, copper, manganese and cadmium in the soil, leaves of fodder plants and in the body parts of the Turkish snail (H. lucorum). The study included ten localities, arranged in four groups, situated in and around the city of Skopje. The first group consisted of five urban localities that were in the central city area. The second group included two suburban localities. The third group consisted of two rural sites. The fourth group represented one site that was located away from the metropolitan area (control site). Soils, leaves of four plant species (Cardaria draba, Lamium purpureum, Rumex crispus and Taraxacum officinale) and a sample of 10 adult snails were collected from each locality. Heavy metals content was determined by wet combustion and atomic absorption spectroscopy. Digestive gland was an organ with the highest amount of all heavy metals and with proven bioaccumulation for Zn and Cd; it showed a prominent correlation with soil concentrations for the four heavy metals but not with the examined plant species. The foot and shell of the snails had limited indicator values since they exhibited weak correlation with soil concentrations of Cu and Mn.

Key words
Digestive gland, foot, shell, heavy metals

How to Cite
Mitev Т., Dedov I.K. & Hristovski S. 2023. Contents of Zn, Cu, Mn and Cd in soil, plants and Turkish snails Helix lucorum Linnaeus, 1758 in Skopje, Republic of North Macedonia. Acta zoologica bulgarica 75 (2) 199-208.

References

  1. Allen S. E. (Ed.). (1989). Chemical analysis of ecological materials. Second edition. Blackwell Scientific Publications, Oxford-London-Edinburgh-Boston-Melbourne, 368 p.
  2. Anttila P., Stefanovska A., Nestorovska-Krsteska A., Grozdanovski L., Atanasov I., Golubov N., Ristevski P., Toceva M., Lappi S. & Walden J. 2016. Characterisation of extreme air pollution episodes in an urban valley in the Balkan Peninsula. Air Quality, Atmosphere & Health 9 (2): 129–141.
  3. Beeby A. & Richmond L. 2002. Evaluating Helix aspersa as a sentinel for mapping metal pollution. Ecological Indicators 1 (4): 261–270.
  4. Berger B. & Dallinger R. 1993. Terrestrial snails as quantitative indicators of environmental metal pollution. Environmental Monitoring and Assessment 25 (1): 65–84.
  5. Ćirić J., Cerić O., Marković R., Janjić J., Spirić D., Popović M., Pećanac B., Baltić B. & Baltić M. 2018. Seasonal distributions of heavy metal concentrations in different snail (Helix pomatia) tissues from an urban environment in Serbia. Environmental Science and Pollution Research 25 (33): 33415–33422.
  6. Coughtrey P. J. & Martin M. H. 1976. The distribution of Pb, Zn, Cd and Cu within the pulmonate mollusc Helix aspersa Müller. Oecologia 23 (4): 315–322.
  7. Cvetkovska-Gjorgievska A., Dedov I., Hristovski S., Langourov M., Lazarevska S., Prelik D. & Simov N. 2019. New records of allochtonous, invasive and pest invertebrate species from the Republic of Macedonia. Ecologica Montenegrina 20: 56–70.
  8. Dallinger R. & Wieser W. 1984. Patterns of accumulation, distribution and liberation of Zn, Cu, Cd and Pb in different organs of the land snail Helix pomatia L. Comparative Biochemistry and Physiology Part C: Comparative Pharmacology 79 (1): 117–124.
  9. de Vaufleury A. G. & Pihan F. 2000. Growing snails used as sentinels to evaluate terrestrial environment contamination by trace elements. Chemosphere 40 (3): 275–284.
  10. Gomot A. & Pihan F. 1997. Comparison of the Bioaccumulation Capacities of Copper and Zinc in Two Snail Subspecies (Helix). Ecotoxicology and Environmental Safety 38 (2): 85–94.
  11. Graham D. L. 1972. Trace metal levels in intertidal mollusks of California. The Veliger 14 (4): 365–372.
  12. Huitson S. B. & Macnair M. R. 2003. Does zinc protect the zinc hyperaccumulator Arabidopsis halleri from herbivory by snails? New Phytologist 159 (2): 453–459.
  13. Ireland M. P. 1984. Seasonal changes in zinc, manganese, magnesium, copper and calcium content in the digestive gland of the slug Arion ater L. Comparative Biochemistry and Physiology Part A: Physiology 78 (4): 855–858.
  14. ISO 2001. Soil Quality: Dissolution for the Determination of Total Element Content. Dissolution with Hydrofluoric and Perchloric Acids. 14869-1. International Organization for Standardization.
  15. Klein-Rollais D. & Daguzan J. 1990. Variation of water content in Helix aspersa Müller in natural environment. Journal of Molluscan Studies 56 (1): 9–15.
  16. Laskowski R. & Hopkin S. P. 1996 a. Accumulation of Zn, Cu, Pb and Cd in the garden snail (Helix aspersa): Implications for predators. Environmental Pollution 91 (3): 289–297.
  17. Laskowski R. & Hopkin S. P. 1996 b. Effect of Zn, Cu, Pb, and Cd on fitness in snails (Helix aspersa). Ecotoxicology and Environmental Safety 34 (1): 59–69.
  18. Lazarevski A. 1993. Climate in Macedonia. Skopje: Kultura. 282 pp.
  19. Marigomez J. A., Angulo E. & Saez V. 1986. Feeding and growth response to cooper, zinc, mercury, and lead in the terrestrial gastropod Arion ater (Linne). Journal of Molluscan Studies 52 (1): 68–78.
  20. Martinez G. S., Spadaro J. V., Chapizanis D., Kendrovski V., Kochubovski M. & Mud P. 2018. Health impacts and economic costs of air pollution in the metropolitan area of Skopje. International Journal of Environmental Research and Public Health 15 (626): 1–11.
  21. Meincke K. F. & Schaller K. H. 1974. Use of snail (Helix pomatia L.) on cultivated land as indicator of environmental-pollution with iron, zinc, and lead. Oecologia 15 (4): 393–398.
  22. Menta C. & Parisi V. 2001. Metal concentrations in Helix pomatia, Helix aspersa and Arion rufus: a comparative study. Environmental Pollution 115 (2): 205–208.
  23. Mitev T., Dedov I. & Hristovski S. 2019. Heavy metals content (Zn, Cu, Mn and Cd) in edible Turkish snail (Helix lucorum L.) in Skopje, Republic of North Macedonia. Macedonian Journal of Ecology and Environment 21 (1–2): 65–71.
  24. Neubert E. 2014. Revision of Helix Linnaeus, 1758 in its eastern Mediterranean distribution area and reassignment of Helix godetiana Kobelt, 1878 to Maltzanella Hesse, 1917 (Gastropoda, Pulmonata, Helicidae). Contributions to Natural History 26: 1–100.
  25. Noret N., Meerts P., Tolrà R., Poschenrieder C., Barceló J. & Escarre J. 2004. Palatability of Thlaspi caerulescens for snails: Influence of zinc and glucosinolates. New Phytologist 165 (3): 763–772.
  26. Noret N., Meerts P., Vanhaelen M., Dos Santos A. & Escarré J. 2007. Do metal-rich plants deter herbivores? A field-test of the defence hypothesis. Oecologia 152 (1): 92–100.
  27. Notten M. J., Oosthoek A. J., Rozema J. & Aerts R. 2006. Heavy metal pollution affects consumption and reproduction of the landsnail Cepaea nemoralis fed on naturally polluted Urtica dioica leaves. Ecotoxicology 15 (3): 295–304.
  28. Pihan F. & de Vaufleury A. 2000. The Snail as a Target Organism for the Evaluation of Industrial Waste Dump Contamination and the Efficiency of Its Remediation. Ecotoxicology and Environmental Safety 46 (2): 137–147.
  29. Rabitsch W. B. 1996. Metal accumulation in terrestrial pulmonates at a lead/zinc smelter site in Arnoldstein, Austria. Bulletin of Environmental Contamination and Toxicology 56 (5): 734–741.
  30. Regoli F., Gorbi S., Fattorini D., Tedesco S., Notti A., Machella N., Bocchetti R., Benedetti M. & Piva F. 2006. Use of the Land Snail Helix aspersa as Sentinel Organism for Monitoring Ecotoxicologic Effects of Urban Pollution: An Integrated Approach. Environmental Health Perspectives 114 (1): 63–69.
  31. Roma A. D., Neola B., Serpe F. P., Sansone D., Picazio G., Cerino P. & Esposito M. 2017. Land snails (Helix aspersa) as bioindicators of trace element contamination in Campania (Italy). OALib 4 (2): 1–12.
  32. Russell L. K., de Haven J. I. & Botts R. P. 1981. Toxic effects of cadmium on the garden snail (Helix aspersa). Bulletin of Environmental Contamination and Toxicology 26 (1): 634–640.
  33. Samiullah Y. 1990. Biological monitoring of environmental contaminants: Animals. MARC report no. 37, GEMS-Monitoring and Assessment Research Centre, King’s College, London. University of London, p. 767.
  34. Scheifler R. 2002. Evaluation de la biodisponibilite et des transferts de pollutants métalliques et organiques dans des réseaux trophiques “sols-plantes-invertébrées.” Thése de doctorat de l’Université de Franche-Comté, spécialité Sciences de la Vie. https://www.osti.gov/etdeweb/servlets/purl/20723685.
  35. Scheifler R., Gomot-de Vaufleury A., Toussaint M. L. & Badot P. M. 2002. Transfer and effects of cadmium in an experimental food chain involving the snail Helix aspersa and the predatory carabid beetle Chrysocarabus splendens. Chemosphere 48 (6): 571–579.
  36. SSO (2022). Population by sex and five year age groups and statistical regions. Transport statistics. State Statistical Office of the Republic of North Macedonia. https://makstat.stat.gov.mk/ (accessed 09.09.2022).
  37. Stafilov T., Šajn R. & Ahmeti L. 2019. Geochemical characteristics of soil of the city of Skopje, Republic of Macedonia. Journal of Environmental Science and Health, Part A 54 (10): 972–987.
  38. Stankovic S. V., Stojkoska E. & Norris A. 2006. Annotated checklist of the terrestrial gastropods (Gastropoda) of the Republic of Macedonia. Anniversary Proceedings (1926-2006). Museum Macedonicum Scientiarum Naturalium: pp. 43-55. Skopje, 2006. UDC 594.3(497.7)
  39. Stenner R. D. & Nickless G. 1974. Absorption of cadmium, copper and zinc by dog whelks in the Bristol Channel. Nature 247 (5438): 198–199.
  40. Sturba L., Fattorini N., Liberatori G., Vannuccini M. L., Nannoni F., Protano G., Tursi A. & Corsi I. 2020. Multi-model inference analysis of toxicological responses and levels of heavy metals in soft tissue of land snail Cornu aspersum caged in proximity to an industrial setting. Ecological Indicators 117: 106688.
  41. Van Gestel C. A. M. & Van Straalen N. M. 1994. Ecotoxicological test systems for terrestrial invertebrates. In M. H. Donker, H. Eijsackers, & F. Heimbach (Eds.). Ecotoxicology of Soil Organisms: pp. 205–208. Boca Raton, FL, USA: CRS Press.
  42. Viard B., Pihan F., Promeyrat S. & Pihan J. C. 2004. Integrated assessment of heavy metal (Pb, Zn, Cd) highway pollution: Bioaccumulation in soil, Graminaceae and land snails. Chemosphere 55 (10): 1349–1359.
  43. Vukašinović-Pešić V., Pilarczyk B., Miller T., Rajkowska-Myśliwiec M., Podlasińska J., Tomza-Marciniak A., Blagojević N., Trubljanin N., Zawal A. & Pešić V. 2020. Toxic elements and mineral content of different tissues of endemic edible snails (Helix vladika and H. secernenda) of Montenegro. Foods 9 (6): 731.