Vol. 76 (1) 2024
ARTICLES
Evaluation of the Effects of Environmental Pollutants
on Populations of the European Pond Turtle Emys orbicularis (Linnaeus, 1758) (Testudines: Emydidae) at Çanakkale, Türkiye
Nurşen Çördük, Çiğdem Gül, Nurcihan Hacıoğlu Doğru & Murat Tosunoğlu*
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*Department of Biology, Faculty of Sciences, Çanakkale Onsekiz Mart University, Çanakkale, Türkiye; mrttosunoglu35@gmail.com
Abstract
We evaluated and compared the effects of different environmental pollutants on haematological, microbiological and genotoxic characteristics of populations of the freshwater turtle Emys orbicularis from the Kavak Delta and Dardanos in Çanakkale (NW Türkiye). The haematocrit values of samples of E. orbicularis differed considerably between the two localities: they were higher in the Kavak Delta samples. The mean cell haemoglobin concentration was also significantly different between the two localities (higher in the samples of the Dardanos). The water samples of the two localities were within the limit (Class I-II) for all parameters, except Dardanos temperature (Class III). Although it was not statistically significant, the nuclear abnormality formation in the erythrocytes of the sample from Dardanos was numerically higher than that from the Kavak Delta. The study assessed the impact of pollutants on populations of Emys orbicularis and conducted on-site evaluations and monitoring of genotoxic pollution.
Key words
Reptilia, Emydidae, genotoxicity, haematology, microbiology
How to Cite
Çördük N. Gül Ç., Hacıoğlu N. Doğru & Tosunoğlu M. 2024. Evaluation of the Effects of Environmental Pollutants on Populations of the European Pond Turtle Emys orbicularis (Linnaeus, 1758) (Testudines: Emydidae) at Çanakkale, Türkiye. Acta zoologica bulgarica 76 (1) 105-114.
References
- Allemen A.R., Jacobson E.R. & Raskin RE. 1999. Morphological, cytochemical staining and ultrastructural characteristics of blood from eastern diamondback rattlesnakes (Crotalus adamanteus). American Journal of Veterinary Research 60(4):507–514.
- APHA 1998. Standard Method for the Examination of Water and Wastewater. 20th Edition, APHA-AWWA-WEF, Washington DC.
- Ballard B.M. & Cheek R. 2003. Exotic animal medicine for the veterinary technician. Blackwell Publishing, Iowa State Press, Ames. 520 p.
- Bhat S.A., Meraj G., Yaseen S. & Pandit A.K. 2014. Statistical assessment of water quality parameters for pollution source identification in Sukhnag stream: An inflow stream of Lake Wular (Ramsar Site), Kashmir Himalaya. Ecosystems, pp. 1–18.
- Bilgin H. & Gül Ç. 2019. Examination of size-dependent hematological parameters in populations of Mauremys rivulata (Testudinata: Geoemydidae) distributed in Canakkale. International Journal of Scientific and Technological Research 5: 27–34.
- Brooks R.J., Brown G.P. & Galbraith D.A. 1991. Effects of sudden increase in natural mortality of adults on population of the common snapping turtle (Chelydra serpentina). Canadian Journal of Zoology 69: 1314–1320.
- Çiçek K., Arikan H. & Ayaz D. 2015. Blood Cells Morphology and Erythrocytes Count of Two Freshwater Turtles, Emys orbicularis and Mauremys rivulata, from Türkiye. Ecologia Balkanica 7(1): 21–27.
- Douglas J.W., Weiss K. & Wardrop J. 2010. Schalm’s veterinary hematology. 6th edition., Iowa 50014–8300, USA: Wiley-Blackwell. 1181 p.
- Ergene S., Çavaş T., Çelik A., Köleli N., Kaya F. & Karahan A. 2007. Monitoring of nuclear abnormalities in peripheral erythrocytes of three fish species from the Goksu Delta (Turkey): genotoxic damage in relation to water pollution. Ecotoxicology 16: 385–391.
- Ficetola G.F., Padoa-schioppa E., Monti A., Massa R., De Bernardi F. & Bottoni L. 2004. The importance of aquatic and terrestrial habitat for the European pond turtle (Emys orbicularis): implications for conservation planning and management. Canadian Journal of Zoology 82(11):1704–1712.
- Gibbons J.W. 1987. Why do turtles live so long?. BioScience 37: 262–269.
- Gidirişlioğlu A.R., Çakir H., Tok H., Ekinci V.E. & Yüksel O. 1998. Ergene Nehri ve kollarının evsel ve endüstriyel atıklarla kirlenmesi ve toprak üzerine etkileri. Köy Hizmetleri Kırklareli Araştırma Enstitüsü Md. Kırklareli, pp. 308–321 (In Turkish).
- Guilherme S., Válegab M., Pereirab M.E., Santosa M.A. & Pachecoa M. 2008. Erythrocytic nuclear abnormalities in wild and caged fish (Liza aurata) along an environmental mercury contamination gradient. Ecotoxicology and Environmental Safety 70(3): 411–421.
- Gül Ç., Tosunoğlu M. & Hacioğlu N. 2015. Some Hematological Parameters of the Mauremys rivulata in Two Different Water Quality in the Biga Stream (Çanakkale, Turkey). ICWERC 2015: XIII International Conference on Wildlife Ecology, Rehabilitation and Conservation 17(4): 1960–1962. Paris, France.
- Hacioglu N. & Dulger B. 2009. Monthly variation of some physico-chemical and microbiological parameters in Biga Stream (Biga, Canakkale, Turkey). African Journal of Biotechnology 8(9):1929–1937.
- Hacioglu N. & Dulger B. 2010. Montly variation of some physico-chemical and microbiological parameters in Saricay stream (Canakkale, Turkey). Fresenius Environmental Bulletin 19(5a): 986–990.
- Hacioğlu Doğru N., Gül Ç., Çördük N. & Tosunoğlu M. 2022. Determination of the effects of environmental pollution on the Balkan terrapin, Mauremys rivulata (Valenciennes, 1833). Acta Veterinaria Hungarica 70 (3): 245–253.
- Hailey A. & Loumbourdis, N.S. 1990. Population ecology and conservation of tortoises: demographic aspects of reproduction in Testudo hermanni. Herpetological Journal 1: 425–434.
- Hailey A. 2000. The effects of fire and mechanical habitat destruction on survival of the tortoise Testudo hermanni in northern Greece. Biological Conservation 92: 321–333.
- Hall R.J. 1980. Effects of environmental contaminants on reptiles: a review. Special Scientific Report-Wildlife 228. U.S. Fish and Wildlife Service, Washington, DC.
- Hidalgo-Vila J., Díaz-Paniagua C., Frutos-Escobar C., Jiménez-Martínez C. & Pérez-Santigosa N. 2007. Salmonella in free-living terrestrial and aquatic turtles. Veterinary Microbiology 119(2–4): 311–315.
- Hofmeyr M.D., Henen B.T. & Walton S. 2017. Season, sex and age variation in the haematology and body condition of geometric tortoises Psammobates geometricus. African Zoology 52(1): 21–30.
- IUCN 2022. IUCN Red List of Threatened Species. Available at: https://www.iucnredlist.org. Accessed on 28 April 2022.
- Josende M.E., Tozetti A.M., Alalan M.T., Filho V.M., Ximenez S., Junior F.M.R. & Martins S. E. 2015. Genotoxic evaluation in two amphibian species from Brazilian subtropical wetlands. Ecological Indicators 49: 83–87.
- Kara C. & Çömlekçioğlu U. 2004. Karaçay (Kahramanmaraş)’ın kirliliğinin biyolojik ve fiziko-kimyasal parametrelerle incelenmesi. KSÜ Fen ve Mühendislik Dergisi 7(1): 1–7 (In Turkish).
- Kelkit A. & Öztürk C. 2005. Kavak Deltası (Çanakkale)’nin korunan alanlar açısından incelenmesi. In: Korunan Alanlar Sempozyumu (8-10 Eylül 2005). Isparta, Türkiye, pp. 233–236 (In Turkish).
- Lambert M.R.K. 1997. Effects of pesticides on amphibians and reptiles in sub-Saharan Africa. Reviews of Environmental Contamination and Toxicology 150: 31–73.
- Lee R.F. & Steinert S. 2003. Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals. Mutation Research 544: 43–64.
- Maceda-veiga A., Figuerola J., Martínez-silvestre A., Viscor G., Ferrari N. & Pacheco M. 2015. Inside the Redbox: Applications of haematology in wildlife monitoring and ecosystem health assessment. Science of the Total Environment 514: 322–332.
- Marques S.M., Antunes S.C., Pissarra H., Pereira M.L., Gonçalves F. & Pereira R. 2009. Histopathological changes and erythrocytic nuclear abnormalities in Iberian green frogs (Rana perezi Seoane) from a uranium mine pond. Aquatic Toxicology 91: 187–195.
- Matson C.W., Palatnikov G., Islamzadeh A., Mcdonald T.J., Autenrieth R.L., Donnelly K.C. & Bickham J.W. 2005. Chromosomal damage in two species of aquatic turtles (Emys orbicularis and Mauremys caspica) inhabiting contaminated sites in Azerbaijan. Ecotoxicology 14: 513–525.
- Meyers-Shöne L. & Walton B.T. 1990. Comparison of two freshwater turtle species as monitors of environmental contamination. Environmental Toxicology Chemistry 12: 1487–1496.
- Napierska D., Barŝienė J., Mulkiewicz E., Podolska M. & Rybakovas A. 2009. Biomarker responses in flounder Platichthys flesus from the Polish coastal area of the Baltic Sea and applications in biomonitoring. Ecotoxicology 18: 846–859.
- Radu V.M., Ionescu P., Deak G.Y., Ivanov A.A. & Diacu E. 2014. Multivariate statistical analysis for quality assessment of aquatic ecosystem on the lower Danube. Journal of Environmental Protection and Ecology 15(2): 412–424.
- Salles F.J., Toledo M.C.B., César A.C.B., Ferreira G.M. & Barbério A. 2016. Cytotoxic and genotoxic assessment of surface water from São Paulo state, Brazil, during the rainy and dry seasons. Ecotoxicology 25: 633–645.
- Simonyan A., Hovhannisyan G., Sargsyan A., Arakelyan M., Minasyan S. & Aroutiounian R. 2018. DNA damage and micronuclei in parthenogenetic and bisexual Darevskia rock lizards from the areas with different levels of soil pollution. Ecotoxicology and Environmental Safety 154: 13–18.
- Strunjak-perovic I., Lisicic D., Coz-rakovac R., Popovic N.T., Jadan M., Benkovic V. & Tadic Z. 2010. Evaluation of micronucleus and erythrocytic nuclear abnormalities in Balkan whip snake Hierophis gemonensis. Ecotoxicology 19: 1460–1465.
- Stuckas H., Velo-antón G., Fahd S., Kalboussi M., Rouag R., Arculeo M., Marrone F., Sacco F., Vamberger M. & Fritz U. 2014. Where are you from, stranger? The enigmatic biogeography of North African pond turtles (Emys orbicularis). Organisms Diversity & Evolution 14: 295–306.
- Sungur A. & Özcan H. 2015. Chemometric and geochemical study of the heavy metal accumulation in the soils of a salt marsh area (Kavak Delta, NW Turkey). Journal of Soils and Sediments 15: 323–331.
- Tanyer G. 1985. Hematoloji ve Laboratuar. Ayyıldız Matbaa A.Ş. pp. 111–122 (In Turkish).
- Tavares-Dias M., Oliveira-Junior A.A., Silva M.G., Marcon J.L. & Barcellos J.F.M. 2009. Comparative hematological and biochemical analysis for giant turtles from the Amazon farmed in poor and normal nutritional conditions. Veterinarski Arhives 79: 601–610.
- Theodorakis C.W., Swartz C.D., Rogers W.J., Bickham J.W., Donnelly K.C. & Adams S.M. 2000. Relationship between genotoxicity, mutagenicity, and fish community structure in a contaminated stream. Journal of Aquatic Ecosystem Stress and Recovery 7: 131–143.
- Thrall A., Baker D.C., Campbell T.W., Denicola D., Feetman M.J., Lassen E.D., Rebar A. & Weiser G. 2004. Veterinary hematology and clinical chemistry. Blackwell Publishing, Philadephia. 776 p.
- Tosunoğlu M., Yilmaz N. & Gül Ç. 2011. Effects of varying ecological conditions on the blood parameters of freshwater turtles in Canakkale (Turkey). Ekoloji 20 (78): 7–12.
- WPCR 1988. Water Pollution Control Regulation (in Turkish), I Section, Official Gazette, 25687, pp. 7–29.
- Yayintaş O.T., Yilmaz S., Türkoğlu M., Çolakoğlu F.A. & Çakir F. 2007. Seasonal variation of some heavy metal pollution with environmental and microbiological parameters in sub-basin Kocabas Stream (Biga, Canakkale, Turkey) by ICP-AES. Environmental Monitoring and Assessment 134: 321–331.
- Yilmaz N. & Tosunoğlu M. 2010. Hematology and some plasma biochemistry values of free-living freshwater turtles (Emys orbicularis and Mauremys rivulata) from Turkey. North-Western Journal of Zoology 6 (1): 109–117.
- Zapataa L.M., Bockb B.C., Orozcoa L.Y. & Palacioa J.A. 2016. Application of the micronucleus test and comet assay in Trachemys callirostris erythrocytes as a model for in situ genotoxic monitoring. Ecotoxicology and Environmental Safety 127: 108–116.
- Zhelev Z., Arnaudova D. & Tsonev S. 2022. Genotoxicity and erythrocyte nuclear abnormalities in Pelophylax ridibundus (Pallas, 1771) (Anura: Ranidae) in an industrial area in southern Bulgaria: Evaluation as biomarkers for ecological stress assessment. Acta Zoologica Bulgarica 74 (1): 59–67.


