Vol. 76 (3) 2024

ARTICLES

Inter-primer Binding Site Markers (iPBS) are Used to Describe the Genetic Diversity of Populations of the Black-striped Pipefish Syngnathus abaster Risso, 1827 (Actinopterygii: Syngnathidae) in their Natural and Expansion Ranges in Ukraine

Yuliia Kutsokon1,2, Yuliia Popovych3, Sabina Chebotar3, Alla Bakuma4, Volodymyr Yuryshynets5 & Yuriy Kvach4,6*

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1Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine, B. Khmelnytskoho St. 15, 01054, Kyiv, Ukraine; 0000-0001-9721-5638
2Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523 Bratislava, Slovakia
3Odesa I. I. Mechnikov National University, Vsevoloda Zmienka St., 2, 65002, Odesa, Ukraine; 0000-0001-6576-7606; 0000-0002-9130-7272
4*Institute of Marine Biology, National Academy of Sciences of Ukraine, Italiyska St. 37, 65048, Odesa, Ukraine; 0000-0001-7190-2210
5Institute of Hydrobiology, National Academy of Sciences of Ukraine, Volodymyra Ivasyuka Av. 12, 04210, Kyiv, Ukraine; 0000-0001-6310-7874
6*Institute of Fisheries, Marine Ecology and Oceanography, Oleksandra Konyskoho St. 82a, 04053, Kyiv, Ukraine; yuriy.kvach@gmail.com; 0000-0002-6122-4150

https://doi.org/10.71424/azb76.3.002803

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Abstract
The black-striped pipefish (Syngnathus abaster) is an Atlanto-Mediterranean marine and estuarine fish species which recently expanded its range into freshwaters. iPBS genetic markers analysis revealed the population divergence of this fish species from different localities in Ukraine. In the course of molecular genetic analysis using four inter-primer binding site (iPBS) primers, 82 amplification fragments were detected. The difference between populations depends on the frequency of a particular allele allows us to separate the population from the Tylihul Estuary and all freshwater ones. The samples from the Dnipro River basin (Stuhna River and Dnipro Reservoir) related to different subclusters, which may indicate that the introduction of the pipefish into the Dnipro basin could have occurred several times and in different ways: 1. Natural range expansion; 2. Artificial acclimatisation; 3. Introduction with river transport. The differences in allele frequencies suggest the existence of populations with different origins in the Dnipro River, which can be explained by the introduction of this species from the delta areas of the Danube and Dniester (population from the Stuhna River) and self-settlement upstream of the Dnipro River (population from the Dnipro Reservoir).

Key words
Syngnathidae, neolimnetics, Dnipro River, Tylihul Estuary, Danube River, Dniester River, tagging

How to Cite
Kutsokon Y., Popovych Y., Chebotar S., Bakuma A., Yuryshynets V. & Kvach Y. 2024. Inter-primer Binding Site Markers (iPBS) are Used to Describe the Genetic Diversity of Populations of the Black-striped Pipefish Syngnathus abaster Risso, 1827 (Actinopterygii: Syngnathidae) in their Natural and Expansion Ranges in Ukraine. Acta zoologica bulgarica 76 (3) 301-307.

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